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The insoluble (pellet) fraction, which contains nuclei, was suspended in ice-cold nuclear extraction reagent and then vortexed at the highest setting for 15 s

Measurements were performed at room temperature under a 480 nm excitation wavelength, a 530 nm emission wavelength, a manual PMT of 500, and a read height of 1.00 mm

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Compression leads to numbness, tingling, weakness, and that distinctive nighttime discomfort many sufferers report

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