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In vivo, the effect is smaller and harder to measure, but skin biopsy studies in trials have shown modest collagen density improvements after weeks of topical use

Patients with greater SQ tissue depth may inject at 90 degrees with a 6 to 8 mm needle

The Journal of clinical investigation 83 , 12471252, doi: 10.1172/JCI114008 (1989)

The choice depends on your syringe size and dosing preferences

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In some cases, waking the individual 10-15 minutes before the night terror is expected to occur and keeping them awake for a few minutes can be an effective way to prevent it