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No controlled human subcutaneous sleep study exists

doi: 10.1002/advs.202404886

(PubMed) Xiong YM, Mo XY, Zou XZ, et al

Heres when caution is advised: Allergy concerns: People allergic to cobalt or B12 should avoid injections

(2) To remove hydrophobic substances, wash the column with 3-4 bed volumes of 70% ethanol or 2 bed volumes of 1% Triton X-100 followed by 5 bed volumes of PBS (pH 7.4) washed