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The focus moved around to different family members and also through time
subtract the blank and plot delta-OD against concentration to obtain the slope
This gives rise to functional iron-deficiency anemia because iron sequestered in these immune cells is unavailable to perform functions vital to the production and maintenance of red blood cells
Interestingly, GSAAT(WT), GSAAT(C138S) and GSAAT(C396S) lines were still markedly redox responsive, and exhibited up to 20% higher enzyme activity when supplied with DTT relative to the UT control conditions, while the two gsa2 lines expressing GSAAT(C168S) and GSAAT(C190S) resulted only in 9% and 2% increased GSAAT activity upon addition of DTT to the extracts ( Supplementary Figure 3E )
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