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Right, volcano plot showing relative fold change (log 2 ) in ER-metabolite abundance versus log 10 (P value) from HEK293T cells expressing a sgRNA targeting intergenic region or sgRNA targeting SLC33A1

Visualization of stained cells was achieved with tyramide-enhanced FITC or rhodamine (1:50 dilution in amplification solution

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They posited that HF induces a disturbance in the metabolism of carbohydrates and fats within myocardial cells, leading to alterations in the cardiac energy metabolic pathway and resulting in the abnormal structure and function of myocardial cells (33)