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The tumor tissue of osteosarcoma was obtained, cut into small fragment, and transplanted subcutaneously into BALB/c nude mice

Barnard ND, Holtz DN, Schmidt N, Kolipaka S, Hata E, Sutton M, et al

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Despite the strong correlation between growth within 3D gels and tumorigenicity [25], it remains unclear why TICs are the exclusive tumor cells capable of growing in this condition, while non-tumor lymphoid cells do not

Previous studies showed that Cd exists in the form of ions or combines with certain components of the cell wall, including glue, cellulose, hemicellulose, and lignin, so that Cd is limited by the cell wall