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If significant fibrosis is confirmed, patients should be referred to a specialist to perform liver biopsy and confirm the diagnosis histologically (59)

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Some researchers transition to maintenance-dose tirzepatide (typically 2.5-5mg weekly) indefinitely rather than stopping completely

The mixture was freeze-thawed twice at 20C for 1 h and 37C for 15 min, the supernatant was obtained by centrifugation at 12000 rpm for 10 min as the whole cell extract