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For phalloidin staining, cells were first permeabilized with 0.1% Triton X 100 in PBS (PBST) for 5 min and then incubated for 2 h with Atto 565 Phalloidin (Sigma-Aldrich # 94072) diluted 1:100 in 1 PBS

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References Practice Committee of the American Society for Reproductive Medicine

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