For example, in Hordeum vulgare leaves challenged with the powdery mildew fungus, Blumeria graminis , the fungal infection led to a significant decrease in APX and GR activity in whole-leaf extracts of resistant variety but caused no significant change in the susceptible one
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For assay of GPX, crude enzyme protein (100 g equivalent) was incubated in assay mixture containing 100 mM potassium phosphate buffer (pH 7.5), 0.5% H 2 O 2 and 0.5 mM ortho-dianisidine
Role in bioactivation and detoxication mechanisms of xenobiotics
Atherosclerosis 152, 307316
While the treatment with NP4 alone caused a strong production of ROS, the preincubation with NAC and subsequent treatment with NP4 showed a poor ROS generation (Fig