For fetal and adult mice intestinal organoids, small intestine sections of approximately 20 cm were harvested, opened longitudinally, rinsed with cold PBS and incubated in 2.5 mM EDTA at 4 C for 30 min with gentle shaking
doi: 10.4161/cam.21054 60 CramerJVBenakisCLieszA
As a subject of scientific study, however, the molecule is decades in the making
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Abnormal TGF-beta-1 signaling degrades the extracellular matrix and stimulates nerve growth factor, potentially increasing pain