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These AG-haESCs were cultured in 2i medium consisting of DMEM (Millipore, Billerica, MA, USA) supplemented with 15% FBS (ExCell Bio, Shanghai, China), penicillinstreptomycin, non-essential amino acids, nucleosides, L-glutamine, -mercaptoethanol, 1000 U/mL LIF, 1 M PD0325901 (Selleck Chemicals, Houston, TX, USA), and 3 M CHIR99021 (Selleck Chemicals)
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Hackford pitched the idea to Jeffrey Katzenberg, who showed interest, the project would end up being distributed through Hollywood Pictures and Buena Vista Pictures, both subsidiaries of The Walt Disney Studios
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