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Briefly, proteins were subjected to sodium dodecyl sulfate-10% polyacrylamide gel electrophoresis (SDS-10% PAGE) under reducing conditions in Laemmlis buffer system followed by transfer to nitrocellulose membranes 19
Interestingly, FSP1 has been redefined as a vital factor dependent on N-myristoylation, which enables FSP1 to anchor in the membrane where FSP1 plays an anti-ferroptosis role by counteracting lethal phospholipid peroxidation dependent on its oxidoreductase activity (Bersuker et al
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