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Stable CHO-SNAP-GLP-1R and MIN6B1-SNAP-GLP-1R cells were generated by transfecting pSNAP-GLP-1R (Cisbio) into wild-type CHO-K1 (ECACC) or MIN6B1, G418 (1 mg ml 1 ) selection, and single-cell sorting by fluorescence-activated cell sorting (FACS) following SNAP-Surface-488 (New England Biolabs) labeling

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One or two cups of coffee is fine, but do not count it toward your daily fluid goal

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