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Alternative repletion strategies have focused on oral administration of GSH precursors (e.g., cysteine and glycine supplementation 9 ), and intravenous administration of GSH, 10 which although promising, is invasive and inconvenient, and therefore unlikely to be a practical solution

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The prepared phosphate buffer was mixed with 0.154 M NaCl solution at a ratio of 1:9 (v/v) and used as phosphate-buffered saline (PBS)

Intracellular metabolites (GSH, glucose, glucose-6P, pyruvate, succinate) were measured by liquid chromatography (Vanquish HPLC Thermo Scientific) coupled to high resolution mass spectrometry (QExactive HF Thermo Scientific)

Bibcode:1998MarER..46...71L