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MDM2 was proven to ubiquitinate the IGF-1R and degraded it in a proteasome-dependent manner (Girnita et al, 2003), eventually leading to cell death

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Several explanations have been proposed to clarify these differences such as different protocols (antibodies, dilutions, antigen retrieval, and cutoff) and the variable frequency of NF1-associated vs sporadic and radiation-associated malignant peripheral nerve sheath tumors between these cohorts

doi: 10.3389/fmed.2020.618506 173 JasimSAAzizDZMustafaYFMargianaRAl-AlwanyAAHjaziAet al

Acceptance criteria are committed in writing before each synthesis run, locking the standard rather than allowing post-hoc adjustment