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Structural figures were prepared using UCSF Chimera 49 and PyMOL (The PyMOL Molecular Graphics System v.2.1)
After incubation for 10 min, 200 L luciferin solution (containing 20 mM tricine, 1.0 mM MgSO4, 0.1 mM EDTA, 33 mM dithiothreitol, 270 M coenzyme A, 500 M D-luciferin, and 530 M ATP, pH 7.8) was added, and then the firefly luminescence was measured with a BioTek synergy 4 Multi-Mode Microplate Reader
A 5mg dose requires 0.29mL
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