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Hu et al., FAK and paxillin dynamics at focal adhesions in the protrusions of migrating cells, Sci
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Migration scratch assay Cells were seeded (60,000 cells/well) in a 96-well plate (Greiner) coated with 0.3 mg/ml matrigel, allowed to attach overnight, and scratched using the IncuCyte Wound maker (Essen Bioscience)
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a RLM (1D): RLM incubation without fractionation, RLM (2D): RLM incubation with fractionation, 3A4 (1D): CYP3A4 Supersome incubation without fractionation, 3A4 (2D): CYP3A4 Supersome incubation with fractionation, DDA: confirmed by DDA, MRM: confirmed by MRM
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