(C) Time-dependent inhibition of TGR activity with GSSG
Recently, it has been confirmed that in the ischemiareperfusion model in rodents, cerebral nerve cell damage is associated with glutathione depletion, and post-ischemia recovery is associated with an increase in the concentration of glutathione (Won et al., 2015)
Subsequently, the cells were washed with PBS and blocked for 1 h with 1% bovine serum albumin (BSA, Sigma A9418) and 22.52 mg/mL glycine in PBST (PBS + 0.05% Tween-20, LPS Solution CBP007T)
10.3389/fcell.2021.705786 189 HuangK
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Active Ingredients: L-Glutathione