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RNA quality was assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, USA), followed by agarose gel electrophoresis and RNA integrity analysis using a 2100 Bioanalyzer (Aligent, California, USA)

Building upon this core machinery, the glucosinolate pathway involves three additional enzymatic modules completing metabolic transformation: (1) CYP83A1 converts aliphatic aldoximes to thiohydroximates, (2) UGT74B1 mediates glycosylation of sulfated intermediates (requiring prior sulfation by SOT17/18), (3) the myrosinase-TGG1/2 system, in concert with epitope-specific proteins (ESPs), hydrolyzes stored glucosinolates into bioactive compounds like SFN upon tissue damage (41)

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Inflammation is a consequence of alpha-synuclein aggregation via an activation of microglia 9 , which, in a prolonged inflammatory state, can result in neurodegeneration