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To induce barrier damage cells were incubated with proinflammatory cytokines (CK) tumor necrosis factor- (TNF-) and interleukin-1 (IL1-) both at 10 ng/ml concentration

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Thyroid panel: TSH, free T4, free T3, and thyroid antibodies

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Competition binding studies using [125I]-IGF-1 demonstrate IC50 values consistent with high-affinity receptor occupancy