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The Fmoc protected amino acids and Fmoc-Lys(C 18diacidmono-t-butyl-y-Glu(AFFA-AFFA)- OtBu)-OH are commercially available or may be prepared according to procedures known in the prior art literature or it may be prepared by the process disclosed in IN 202141019461

MOTS-c (Mitochondrial Open Reading Frame of the 12S rRNA- c) is a small peptide comprised of 16 amino acids, which is encoded by DNA not in the nucleus, but inside the mitochondrial genome, specifically inside the gene that encodes the mitochondrial 12S ribosomal RNA