platensis cells were high-pressure frozen using a Leica EM PACT2 instrument (Leica Microsystems, Buffalo Grove, IL), cryo-substituted for 80 h at 87 C in acetone containing 2% osmium tetroxide, and, after slowly warming to RT, embedded in Spurrs resin
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The constant production of pro-inflammatory cytokines creates a hostile cellular environment that damages the delicate inner mitochondrial membrane, specifically targeting cardiolipina unique lipid essential for anchoring the electron transport chain complexes
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However, with increasing research, the pleiotropic effects of the carnitine pool have gradually emerged