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For CBB staining, SDS-PAGE gels were stained for 10 min using staining solution (0.1% Coomassie Brilliant Blue G250, 10% acetic acid, 50% methanol, and 40% H 2 O 2 ) and destained with distilled water overnight

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Before the promising therapeutic impact of miRNA can be realized, there are several challenges to be addressed, which include but not limited to targeted delivery of oligonucleotides to specific organs, tissues and cell types, poor bioavailability, limited tissue permeability ( via imperfect matching with 3UTRs ( REGULATION OF miRNA EXPRESSION Recently emerged question is how miRNAs are regulated under certain condition such as diseases

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The blend can be legally supplied for laboratory research purposes only