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After hybridization, the non-hybridized PCR products were washed and alkaline phosphatase-labeled streptavidin (conjugate) was bound to the biotinylated hybrid

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Moreover, a recent study from Guzman and colleagues (39) has shown that, in patients with T2D, treatment with a selective GCGR antagonist, LY2409021, induces a significant increase in hepatic lipid content assessed by magnetic resonance imaging, suggesting that GCGR activation is required to prevent build-up of fat in the hepatocytes