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Metabolites were resolved across a 2.1 150 mm, 1.7 m Kinetex C18 column (Phenomenex) using a 5-min, reverse-phase gradient from a previously described method 19

It has been suggested that the Bcl-2 gene product inhibits apoptosis by interacting with mitochondrial Super oxide dismutase (SOD) [22]

OXPHOS complexes activities and the contents of ATP, ROS and acetyl-CoA were then measured in the supernatants using MitoTox Complex I-V kits (abcam, #ab109903, #ab109904, #ab109905, #ab109906, #ab109907), ATP colorimetric/fluorometric assay kit (abcam, #ab83355) and PicoProbe Acetyl-CoA assay kit (abcam, #ab87546) according to their manufacturers instructions

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