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The chromatographic eluent phases were acetonitrile (LC-MS grade, Merck) as phase A and 20 mM ammonium formate in H 2 O (pH 3.5) as phase B

All embryos were cultured using sequential culture medium (Vitrolife, Gothenburg, Sweden) in incubators at 37C under 5% O 2 , 6% CO 2 , and 89% N 2 conditions with high moisture until day 6