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Mortazavi, Seyyed Ali Mohammadi Vadoud, Hamid Reza Moghimi Journal: BioImpacts : BI Journal ranking: Q2 Key takeaways: GHK-Cu and Pal-GHK are effective anti-wrinkle peptides with potential for increased skin permeability through permeation enhancement methodologies

However, it may take a number of days or weeks before a person notices improvements in their symptoms

Ingredients Water, ethylhexyl palmitate, squalane, stearic acid, Copper Tripeptide-1 (GHK-Cu), methylsulfonylmethane, dimethyl MEA (DMAE), cetyl alcohol, glyceryl stearate, PEG-100 stearate, nannochloropsis oculata extract (with pullulan, aqua, phenoxyethanol, and sodium benzoate potassium sorbate), PPG 2 myristyl ether propionate, phenoxyethanol, C10-30 cholesterol/lanosterol esters, polysorbate 80, allantoin, leucine, lavandula angustifolia (lavender) oil, aloe barbadensis (aloe vera) powder, sodium salicylate, vitamin E TPGS (tocophersolan), vitamin E (as d-alpha tocopherol plus mixed tocopherols: d-beta, d-delta and d-gamma tocopherol)

Theoretical concerns include: GHK-Cu angiogenic properties potentially conflicting with calcineurin inhibitor mechanisms

Population Doubling Level Calculation To calculate the PDL, a formula was used as follows: n = 3.32 (log UCY log I ) + X , where n is the final PDL at the end of a given subculture, UCY is the cell yield at that point, I is the cell number used as inoculum to begin that subculture, and X is the PDL of the inoculum used to initiate the subculture being quantitated ( SA--Galactosidase Staining Cells were seeded in 6-well microplates at 4 10 4 cells/well