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For elution of the target protein, the interaction to the ligand is resolved by changing the buffer conditions, for example, the pH value, or by adding a specific competitor that supplants the target protein from the ligand resulting in dissociation of the target, while the ligand remains immobilized on the stationary phase

Solmi M, Veronese N, Manzato E, et al

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Most protocols run 8-12 weeks, followed by 4-8 weeks off

From cardiovascular support to mental clarity to muscle maintenance, the benefits go well beyond the scale