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The membrane was blocked with 3% non-fat milk (BioRad, Hercules, CA, United States) at room temperature for 1.5 h, washed, and incubated with primary antibodies (Supplementary Table 3) at 4C overnight on a rotating shaker

Minimize time outside refrigeration, disinfect stoppers before every use, and use new sterile needles for each draw to maximize viable storage time

Expert Treatment A quick injection is administered into the upper arm or thigh

Zhang L, Gu B, Wang Y (2021a) Clove essential oil confers antioxidant activity and lifespan extension in C

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Its also possible that a crash follows the initial boost of energy as the body adjusts to the sudden influx of B12