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doi:10.1016/j.ab.2003.07.023

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the present invention is related to a process for producing semapeptide, the process comprising the steps of: a) culturing a host cell comprising a nucleotide sequence encoding of Formula (II) under suitable conditions for expression, insoluble tag is a nucleotide sequence of Alanine -Valine

General cell culture and imaging The HeLa cell line used in this study was purchased from American Type Culture Collection (ATCC) and grown in DMEM (Gibco, 11965) media supplemented with 10% FBS and 1% 1003 Pen Strep (Gibco)