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Immunofluorescence staining Fibroblasts were fixed, permeabilised, blocked, and incubated overnight at 4 C with primary antibodies targeting p53-pS15 and FOXO4 shown in Table 2, and developed using Alexa Fluor 488-conjugated donkey anti-mouse immunoglobulin G (1:100

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Obesity is a major driver of metabolic dysfunction, and weight loss alone can significantly improve insulin sensitivity and reduce the risk of chronic disease

Since ALCAR has shown benefits for nerve pain in other conditions, it could be a game-changer for fibromyalgia-related pain as well

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Humanized liver mice Human liver chimeric PXB mice were purchased from PheonexBio, and in-life procedures were performed at InterVivo Solution in accordance with their bioethical guidelines, which are fully compliant with ethical regulations and internationally accepted principles for the care and use of laboratory animals