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& Jozefczak, M

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After incubation, the supernatant was discarded and cold ECM gel from Engelbreth-Holm-Swarm murine sarcoma diluted to 0.35 mg/mL in incubation medium (William's E medium with 0.1 M dexamethasone in DMSO, 0.5% penicillin/streptomycin, 6.25 g/L human recombinant insulin, 6.25 g/mL human transferrin, 6.25 ng/mL selenous acid, 1.25 mg/mL bovine serum albumin, 5.35 g/mL linoleic acid, 2 mM GlutaMAX, and 15 mM HEPES at pH 7.4) was applied over the cells prior to an overnight incubation at 37C

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