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After 20 days of culture, 14 bullfrogs were taken from each tank separately and executed by double destruction of medulla

Arrows indicate the cleavage sites in the native GLP-1, while blocked arrows indicate amino acid modifications (A-Ala, K-Lys, S-Ser, G-Gly, Q-Gln, N-Asn, D-Asp, C-Cys) to prevent cleavage by DPP-IV and trypsin