These results indicate no statistically significant difference between the experimental and saline groups, likely due to the limited sample size
Cutting corners on reconstitution technique or washout duration doesn't save time
The scRNA/CITE-seq libraries were prepared using the GemCode Single Cell 3 Gel Bead and Library kit, version 3 (10x Genomics 1000128) according to the manufacturers instructions with the addition of amplification primer (3 nM, 5CCTTGGCACCCGAGAATT*C*C) during cDNA amplification to enrich the TotalSeq-A cell surface protein oligos
At baseline, trained nurse interviewers administered a structured questionnaire to each subject to collect comprehensive information on demographics, occupation, lifestyle habits, medical history, usage of medication and dietary supplements, alcohol consumption, and past cigarette smoking history
Some people experience headaches, restlessness, and difficulty sleeping
N.TsutsuiT.TamaokiD.SatoK.NishiuchiT