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Full geometry optimizations, relaxed scans and transition structure searches were carried out with Gaussian 16 83 with the ONIOM 84 QM/MM method using the B3LYP hybrid functional 85 and 6-31 G(d) basis set with ultrafine integration grids for the QM part and the ff14SB 78 , gaff2 79 and TIP3P 86 force fields for the MM part (protein, substrate and water, respectively) with electrostatic embedding
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However, when APEX is expressed at low levels, it becomes challenging to detect diaminobenzidine (DAB) signals by electron microscopy (EM), and biotin-phenol activity is difficult to detect in proteomic analysis
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and (3) to illustrate the potential adaption mechanism in HT stress
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