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Cell pellets were gently resuspended in 400 L of lysis buffer (10 mM Tris, pH 8.0, 140 mM NaCl, 1.5 mM MgCl 2 , and 0.5% Igepal) containing 1 protease inhibitor cocktail (Cat# 11697498001

The sweet spot seems to be 3-5 weekly sessions to really establish those cumulative benefits

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