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Cell pellets were washed twice with PBS (Fisher Scientific) and stored at -80C prior to further analysis

Slowing the ramp to the label cadence (2.5 mg start, 2.5 mg steps, four-week holds) dropped that to mid-single digits at every maintenance dose, at no cost to the 12-week result, and the dedicated slow-titration trial reached placebo range. Reaching 15 mg is not the problem

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Alternatively, alternate between abdomen and thigh

Statistical analyses revealed significant interactions between treatment and time for the evaluated vitamins and minerals, indicating differential effects between the two supplements