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At each time point, 1/10 volume of culture medium per well CCK-8 solution reagent was added, incubated at 37C for 3 h, and the absorbance was measured at 450 nm using a microplate reader

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1 Ensure hands and work area are clean before handling any peptide product 2 Topical GHK-Cu products are typically pre-formulated follow product instructions 3 Apply a thin layer to clean, dry skin in the target area 4 Store as directed on packaging, typically at room temperature away from direct sunlight 5 Avoid applying to broken or irritated skin unless directed by a provider Week 12 Initial effects and adaptation period