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5Block, probe, detect, and normalize Block with 5% non-fat dry milk in TBST unless the antibody datasheet recommends another blocker
We used a lag of 6 months for all exposures to minimise protopathic bias and allow for a minimum and sufficient latency period after cohort entry [17, 18]
The general workflow of the developed IGF-1 MSIA-SRM ( Figure 1 ) begins by preparing plasma samples to efficiently disrupt the IGF-1/IGFBP complex and maintain this dissociation
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The American Diabetes Association
Barakat M, DiPietro LA, Chen L
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