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When shortages resolve, compounding pharmacies lose the legal basis to prepare these medications under 503A
1000
Briefly, cells were washed with PBS and changed to media with no serum + 50 g/mL cycloheximide to inhibit protein synthesis for 1 hour, then stimulated with vehicle or 100 nM exendin-4 in reverse time order for 8, 6, 4, 2 and 1 hours
2APB was dosed at 1 or 20 mg/kg and administered to mice 2 h before treatment with 500 mg/kg APAP
There were no hepatic safety concerns identified in the completed trials
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