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A study conducted at the University of Texas Southwestern Medical Center found that NNMT inhibition in obese mice reduced body weight by 7% over four weeks without changes in food intakea reduction driven entirely by increased energy expenditure

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This represents the most common starting point in practical protocols and aligns with the midpoint of clinical dosing ranges adjusted for average body composition

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[16] The principal AOD9604 studies cited here did not establish a complete AOD9604-to-UCP1 pathway or demonstrate direct induction of UCP1 transcription, UCP1 activity, white-adipose browning, or functional thermogenesis

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