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Cai, M., Tejpal, S., Tashkova, M., Ryden, P., Perez-Moral, N., Saha, S., Garcia-Perez, I., Serrano Contreras, J

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Beads were washed with 20 mM and 40 mM imidazole steps and proteins were eluted using 250 mM imidazole in 50 mM Tris-HCl, 100 mM NaCl, 0.5 mM MgCl 2 (pH 7.5)