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Analytical Identification and Purity Research-grade GHK-Cu is typically characterized by HPLC purity 98%, confirmed by reversed-phase chromatography (C18 column, acetonitrile/water gradient with 0.1% TFA)

The major obstacles to developing probes that can monitor GSH dynamics include the reaction reversibility and kinetics of the sensing reaction, as well as the high abundance of GSH (110 mM) inside cells

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