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The animals were anesthetized by inhalation route with Isofluorane (Isboa Vet) using a vaporizer and intranasal administration was performed with two instillations of 20 l of the bacterial suspension prepared in PBS

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Al, obese Zucker rats were received a dose of 500 micrograms/kg of body weight over the course of 19 days

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Department of Health and Human Services found that anorectics like phentermine had among the lowest drug abuse rates per 100,000 emergency room visits lower even than ibuprofen abuse cases