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Hyperpigmentation disorders
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Soluble collagen in the supernatant was also analyzed by western blotting using an specific anti-collagen 1 type I antibody (sc-8784, Santa Cruz, Dallas, Texas) upon protein fractionation in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) following protocols previously described 45
Salabei, J
Since endotoxin (LPS) causes many of its effects indirectly by inducing cytokines, studies were undertaken to determine whether LPS might also induce splenic PTHrP expression
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