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After pelleting at 1000 g for 30 s, the worms were incubated in a solution of 20% bleach (sodium hypochlorite), 5% NaOH, and 75% HO with gentle shaking until the adults dissolved, leaving only eggs behind

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The model is based on data from both in vitro measurements on isolated adipocytes, and in vivo microdialysis measurements, in both cases from non-diabetic individuals