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The optimized MS parameterswere set as follows: Desolvation temperature 400 C, desolvation gas flow 600 L/h, source temperature 110 C, cone gas flow 50 L/h, capillary voltage 3000 V, cone voltage 30 V

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It has been reported that IGFBP-3 binds to a nuclear receptor, 9-cis retinoic acid receptor-alpha (RXR-), which interacts with peroxisome proliferator activated receptor-gamma (PPAR-), a nuclear protein involved in the regulation of glucose and lipid metabolism [175, 176]

These MC1R polymorphism studies have contributed to understanding the molecular basis for the phenotypic consequences of loss-of-function MC1R variants in skin cancer risk and pigmentation biology