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The benefits and drawbacks of glutathione IV include rapid peak concentration within minutes, skin improvement in 37.5% of participants versus 18.7% placebo, and FDA approval as chemotherapy adjunct, while risks encompass adverse events in 32% of users, lack of FDA approval for skin lightening, and benefits fading after 6 months of discontinuation

introduction of two random mutations of Y444A and G484A to D433N resulted in up to 50-fold increase in catalytic efficiency for GL-7-ACA substrate (Yamada et al., 2008)