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Metabolic pathway exploration: Evaluation of GH-associated signaling processes

The homogenates were centrifuged at 20,400 g for 20 min at 4 C, and the resulting supernatants were appropriately diluted and used in the assays, which were conducted according to the manufacturers instructions

GSH deficiency induced in cultured hepatocytes caused an increase in oxidative stress and alterations in VD regulatory genes

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