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For intracellular staining of phospho(p)-mTOR (Fisher Scientific 15549836) and p-S6 (eBioscience 15528216), cells were first stained for extracellular markers, fixed in 2% FA at RT, and stained for p-mTOR and p-S6 diluted 1:200 in saponin 5 g/mL

10.1038/s41598-017-12292-9 7 ArefiS.KhaliliG.IranmaneshH.FariftehF.HosseiniA.FatemiH

Urol.,1968, 99, 980-985 20

Fiber photometry to measure bulk activity of a region (NAc, VTA, prefrontal) during a memory paradigm

While the preclinical safety profile appears favorable (few adverse effects reported in animal studies), this does not guarantee the same in humans, especially with prolonged use

Building on the evidence of Erastin-induced cell death mentioned earlier, we posit that Erastin can restrain lesion growth in EMS by inhibiting system Xc , depleting cystine and GSH, compromising GPX4 activity, and driving lipid peroxidation within EESCs